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Reagents

Published on 26/05/2014

  The EURL can provide the following products: PRODUCT NAME CONDITIONNING RABIES DIAGNOSIS AND TYPING Reagent Polyclonal conjugate 3 ml (1vial per laboratory) Negative Control Non infected red fox brain 1 ml of lyophilised brain Fixed virus strain CVS 27 1 ml of lyophilised brain or 0.5 ml stabilized in RNA later Wild virus strains GS7 (French fox) 1 ml of lyophilised brain or 0.5 ml stabilized in RNA later Raccoon dog (Poland) 1 ml of lyophilised brain or 0.5 ml stabilized in RNA later Ariana (Tunisian dog) 1 ml of lyophilised brain or 0.5 ml stabilized in RNA later Imported dog strain (from Morocco) 1 ml of lyophilised brain or 0.5 ml stabilized in RNA later DUVV 1 ml of lyophilised brain or 0.5 ml stabilized in RNA later EBLV-1a 1 ml of lyophilised brain or 0.5 ml stabilized in RNA later EBLV-1b 1 ml of lyophilised brain or 0.5 ml stabilized in RNA later EBLV-2 1 ml of lyophilised brain or 0.5 ml stabilized in RNA later ABLV 1 ml of lyophilised brain or 0.5 ml stabilized in RNA later FOLLOW UP of ORAL VACCINATION: Age and tetracycline determination CD Rom Protocol Explanation Tooth sample Positive for tetracycline Tooth section or entire tooth Negative for tetracycline Tooth section or entire tooth OTHER Positive control CVS 11 750 µl of suspernatant lyophilised   Please contact the EURL for rabies for any order.   Last update: 09 August 2017

Strain database presentation

Published on 26/05/2014

Strain database The EURL has developed a database recording epidemiological information on rabies strains including the corresponding Genbank sequence accession number. Linking Genbank numbers to this database will avoid the duplication of information and prevent redundancies for the NRLs since the GenBank database is an appropriate and useful tool already used for publication, research and analysis of sequences. The advantage of the EURL's sequence database is that it will make epidemiological information related to this sequence available. Submit cases Please participate actively in the deployment of the database by filling out the form for each of your new positive cases! Submit data on-line Download strain identification form (pdf, to be sent by mail or email) Consult data All data - strain database direct consultation Advanced search in database

Contact

Published on 26/05/2014

  For any questions or remarks regarding this website, or to suscribe to the newsletter, please contact us by email, specifying: “rabies” in the object your name, position and laboratory details in the message. Messages without “rabies” in the subject heading will be destroyed without being read.

Map and directions

Published on 26/05/2014

  ANSES - Nancy laboratory for rabies and wildlife Laboratoire de la rage et de la faune sauvage de Nancy Technopole Agricole et Vétérinaire - Bâtiment H CS 40009 F-54220 MALZEVILLE FRANCE Phone: +33 (0)3 83 29 89 50 Fax: +33(0)3 83 29 89 58 Laboratory access map  

Links

Published on 26/05/2014

  EURL FRANCE - French Agency for Food, Environmental and Occupational Health and Safety (Anses)  www.anses.fr   European NRLs AUSTRIA - Austrian Agency for Health and Food Safety (AGES) www.ages.at BELGIUM - Institut Scientifique de Sante Publique (WIV-ISP)  www.wiv-isp.be CROATIA - Croatian Veterinary Institute www.veinst.hr/en CYPRUS - Veterinary services, Animal Health Laboratory www.moa.gov.cy CZECH REPUBLIC -  Státni Veterinárni Ústav Praha (State Veterinari Institute Prague) www.svscr.cz DENMARK - Technical University of Denmark www.dtu.dk ESTONIA - Estonian Veterinary and Food Laboratory (VFL) www.vetlab.ee FINLAND - Finnish Food Safety Authority Evira www.evira.fi/portal/en GERMANY - Friedrich Loeffler Institut  www.fli.de/en IRELAND - Central Veterinary Research Laboratory www.agriculture.gov.ie/virology ITALY - Istituto Zooprofilattico Sperimentale delle Venezie (IZSVe) www.izsvenezie.it LATVIA - Institute of Food Safety, Animal Health and Environment „BIOR” www.bior.gov.lv LITHUANIA - National Food And Veterinary Risk Assessment Institute www.nmvrvi.lt NETHERLANDS - Central Veterinary Institute of Wageningen UR www.cvi.wur.nl POLAND - National Veterinary Research Institute PIWet www.piwet.pulawy.pl PORTUGAL - Laboratório  Nacional de Investigação Veterinária (LNIV) www.iniav.pt/index.php SLOVAKIA - State Veterinary Institute Zvolen www.svps.sk/english/SVFIZV.asp SWEDEN - SVA www.shttp://www.sva.se/enva.se UNITED KINGDOM - Veterinary Laboratories agency (HAVLA) www.defra.gov.uk/ahvla   International organisations OIE - World Organisation for Animal Health www.oie.int WHO - World Health Organisation www.who.int European Commission www.ec.europa.eu European Commission programmes www.ec.europa.eu EFSA - European Food Safety Authority www.efsa.europa.eu EDQM - European Directorate for the Quality of Medicines and Healthcare www.edqm.eu ICONZ - Integrated Control of Negleted Zoonoses www.iconzafrica.org/ GARC - Global Alliance for Rabies Control www.rabiescontrol.net RABMED CONTROL www.rabmedcontrol.org PREMEDICS www.predemics.biomedtrain.eu Discontool www.discontools.eu SEARG www.searg.info/doku.php (not updated anymore)   Others Rabies bulletin Europe www.who-rabies-bulletin.org ELIZ - (Entente de Lutte Interdépartementale contre les Zoonoses) www.ententeragezoonoses.com World rabies day www.worldrabiesday.org

European Commission rules

Published on 26/05/2014

  .. EURLs, what for? .. In the context of the EU strategy aimed at improving animal health and establishing the single market for live animals and animal products, a network of European Union and National Reference Laboratories dealing with major animal diseases has been gradually set up. The designation of European Union Reference Laboratories should contribute to a high quality and uniformity of analytical results. EURLs for animal health and zootechnics are listed in Annex VIII of Regulation (EC) No 882/2004 . Functions and duties of the EURLs consist notably of providing information on analysis methods and organising comparative tests with the National Reference Laboratories, coordinating the application of the methods and research into new analysis methods, organising training and advanced courses for National Reference Laboratory staff and providing scientific and technical assistance to the Commission. Apart from these duties and functions the Commission approves an annual working plan for each EURL specifying their tasks for the next year. .. Financial aid to EURLs .. Within the framework of Council Decision 90/424/EEC on expenditure in the veterinary field of 26 June 1990, these laboratories may receive European Union financial aid for fulfilling the tasks and functions specified in the animal health legislation. For this reason, the work programmes for the EURLs concerned are approved every year via specific Decisions, based on Council Decision 90/425/EEC. Appropriations shall be decided upon each year as part of the budgetary procedure. Commission Regulation (EC) No 1754/2006 of 28 November 2006 lays down detailed rules for the granting of European Union financial assistance to European Union Reference Laboratories for feed and food and the animal health sector pursuant to Article 28 of Decision 90/424/EEC. The said Regulation provides that the financial contribution from the European Union is to be granted if the approved work programmes are efficiently carried out and that the beneficiaries supply all the necessary information within certain time limits. In accordance with Article 2 of this Regulation, the relationship between the Commission and each European Union Reference Laboratory is laid down in a partnership agreement which is supported by a multi-annual work programme. .. EURL evaluation .. The main purpose of the evaluation is to assess the performance of the EURLs during the past 15 years, especially as regards effectiveness and efficiency and to identify areas for improvement to ensure high quality and harmonisation of laboratory testing of animal diseases across the Community, taking into account the current animal health situation in the EU. The EURL evaluation campaign was held in first quarter 2009. Since the rabies EURL was designated just before the evaluation campaign began (July 2008), it was not able to be evaluated. .. Eradication and monitoring programmes .. The European Commission has eradication and monitoring programmes for animal diseases. These programmes aim to progressively eliminate animal diseases that are endemic to certain areas of the European Union. They also include checks aimed to prevent zoonoses. The programmes include a wide range of measures including vaccination, testing of animals, compensation for slaughtering or culling and treatment. Rabies is part of the second group of diseases (“zoonoses or epizootics not covered elsewhere measures on a herd or flock basis”) covered by the EC programmes. .. Task force for monitoring disease eradication in the Member States .. The Task Force was created in a meeting held on 15 March 2000 in Brussels in accordance with action 29 of the White Paper on Food Safety . The objectives of the Task Force are to improve animal disease eradication as well as improve the cost-benefit ratio of animal disease eradication programmes co-financed by the European Union. Meetings are chaired by the Commission and take place in Brussels each three to six months. For some diseases (sheep and goat brucellosis, bovine brucellosis, bovine tuberculosis, rabies, salmonellosis) subgroups of the Task Force have been established. The subgroups support the Task Force and the Member States in their attempts to develop and to implement optimal disease eradication measures. The members of the subgroups are personally nominated. For each meeting of the Subgroups a final report is produced. In those reports, a number of conclusions and recommendations are provided. The Task Force for rabies was created in 2004. Year Date Town Country 2015 15-16 October Budapest Hungary 2012 11-12 December Warsaw Poland 2012 29-30 November Zagreb Croatia 2011...

EU National Reference Laboratories for rabies

Published on 26/05/2014

List of the EU National Reference Laboratories for rabies, including presentation (for private members only) and official website:   AUSTRIA Presentation: Austrian NRL Official website: Austrian Agency for Health and Food Safety (AGES), Institute for Veterinary Disease Control   BELGIUM Presentation: Belgian NRL Official website: Sciensano   BULGARIA Presentation: Bulgarian NRL Laboratory: National Diagnostic Research Veterrinary Medical Institute (NDRVMI)   CROATIA (Republic of) Presentation: Croatian NRL Official website: Croatian Veterinary Institute   CYPRUS Presentation: Cypriot NRL Official website: Animal Health Laboratory - Virology section    CZECH REPUBLIC Presentation: Czech NRL Official website: State Veterinary Institute Prague         DENMARK     Presentation: Danish NRL Official website: DTU Vet    ESTONIA     Presentation: Estonian NRL Official website: Estonian veterinary and Food laboratory   FINLAND Presentation: Finnish NRL Official website: Finnish Food Safety Authority Evira    FRANCE     Presentation: French NRL and EURL Official website: Anses-Nancy Laboratory for rabies and wildlife    GERMANY Presentation: German NRL Official website: Friedrich-Loeffler-Institut - Federal Research Institute for Animal Health    GREECE Presentation: Greek NRL Laboratory: Virology Laboratory, Department of Molecular Diagnostics, FMD, Virological, Ricketsial and Exotic Diseases, Athens Veterinary Center    HUNGARY     Presentation: Hungarian NRL (sorry, no file) Official website: Veterinary Diagnostic Directorate, National Food Chain Safety Office    IRELAND Presentation: Irish NRL Official website: Central Veterinary Research Laboratory (CVRL), Department of Agriculture, Food and the Marine    ITALY Presentation: Italian NRL Official website: Istituto Zooprofilattico Sperimentale delle Venezie (IZSVe)    LATVIA Presentation: Latvian NRL Official website: Institute of Food Safety, Animal Health and Environment BIOR    LITHUANIA Presentation: Lithuanian NRL Official website: National Food and Veterinary Risk Assessment Institute    LUXEMBOURG Presentation: Luxembourg NRL (sorry, no file) Official website: Laboratoire de Medecine Veterinaire de L'Etat (in French)    MALTA Presentation: Maltese NRL (sorry, no file) Official website: Veterinary and Phytosanitary Regulation Department, Veterinary Regulation Directorate    THE NETHERLAND     Presentation: Dutch NRL Official website: Central Veterinary Institute of Wageningen UR    POLAND Presentation: Polish NRL Official website: National Veterinary Research Institute - Department of Virology    PORTUGAL      Presentation: Portuguese NRL (sorry, no file) Official website: INIAV, Instituto Nacional de Investigação Agrária e Veterinária, I.P. (In Portuguese)    ROMANIA     Presentation: Romanian NRL Official website: Institute for Diagnosis and Animal Health Bucharest - Virology Department (in Romanian)    SLOVAKIA     Presentation: Slovak NRL Official website: State Veterinary and Food Institute - Veterinary Institute Zvolen    SLOVENIA     Presentation: Slovenian NRL Official website: National veterinary institute    SPAIN     Presentation: Spanish NRL Official website: Instituto de Salud Carlos III, Centro Nacional de Microbiología    SWEDEN     Presentation: Swedish NRL Official website: SVA, National Veterinary Institute    UNITED KINGDOM Presentation: British NRL Official website: Animal & Plant Health Agency Weybridge     Last update: 08 August 2017

Other international activities

Published on 26/05/2014

Last update: 20 November 2014   The laboratory is: WHO Collaborating Centre for Research and Management in zoonosis control since September 1983, OIE Reference Laboratory for rabies since June 1991, European Union Reference Laboratory for rabies serology since March 2000, European Union Reference Laboratory for rabies since July 2008. The laboratory provides scientific and technical support at the European level (European Commission, EDQM, EFSA), to international organizations (OIE, WHO) through numerous activities. The laboratory works closely to the National Reference Laboratories and their competent authorities for defining the strategy and the organization of oral vaccination programmes, as well as for establishing the surveillance and the monitoring of the disease (incidence of rabies, assessment of bait uptake and of rabies immunity both in adult and young target animals). The laboratory ensures training activities in the frame of its own programmes, within the limit of its possibilities and collaborates in the preparation and distribution of teaching materials. Biological reference material and laboratory services are available on request to help international laboratories to develop their own rabies surveillance and monitoring systems, as well as any other laboratory services. The rabies laboratory's experts provide scientific and technical assistance, advice and training for Member States and third countries. They are also involved in technical studies for improving and adapting existing diagnostic, serological and molecular biology methods. .. Current projects .. ICONZ   ICONZ (FP7-KBBE-2007-1-3-09) Integrated control of neglected Zoonoses: Improving human health and animal production through scientific innovation and public engagement. ICONZ is a consortium of 22 partners from 18 countries. The project focuses on the neglected zoonoses found in Africa and contributes to the improvement of animal and human health. Neglected zoonoses such as anthrax, rabies, brucellosis, bovine tuberculosis, zoonotic trypanosomiasis, echinococcosis, cysticercosis and leishmaniasis are the targeted diseases are being currently investigated. The ultimate objective is to develop and promote integrated controls for neglected zoonoses in developing countries.   PREDEMICS   Preparedness, Prediction and Prevention of Emerging Zoonotic Viruses with Pandemic Potential using Multidisciplinary Approaches. The project is studying four representative RNA viruses with potential for crossing the species barrier (influenza viruses, Hepatitis E virus, Japanese encephalitis virus and related flaviviruses, as well as lyssaviruses), especially those causing rabies. The project involves the participation of veterinary and public health scientists from 17 European institutes. The main objective is to investigate in depth the interactions between the virus and its host to determine the factors linked to the emergence of the diseases.     Additional documentation .. OIE  (World Organisation for Animal Health) WHO (World Health Organisation) ICONZ PREDEMICS

Work on rabies by Nancy laboratory for rabies and wildlife

Published on 26/05/2014

  ..   Organising, coordinating, implementing and monitoring oral fox vaccination campaigns against rabies .. In the 1970s, the laboratory extensively participated to scientific research work for developing oral vaccine baits. A number of experimental trials were undertaken to check efficacy and safety of vaccines before their marketing, in collaboration with other international groups involved in rabies activities. In 1986, the laboratory tested the first available methods of oral vaccination with baits (chicken head baits) deposited in the field by foot distribution. From 1990, the overall organisation of the oral vaccination campaigns has been centralised: the Anses-Nancy Laboratory for rabies and wildlife was requested by the national competent authority to organize and plan the oral vaccination of foxes and, in close cooperation with its delegates, to implement and evaluate the programmes. From 1988 to 1998, year of the last recorded case of vulpine rabies in France, the laboratory planned the strategy and performed oral vaccination campaigns in infected areas twice a year using helicopters. These campaigns were then prolonged until 2003 along Northern and Eastern borders and were performed once again in 2005. Several field trials have been conducted to improve the efficiency of oral vaccination programme, particularly to promote cubs' coming into contact with baits and also to counterbalance the increase in the fox population.   Rabies surveillance and vaccination monitoring .. Rabies surveillance is the key element of rabies control and prevention programmes. It is based on laboratory investigations on samples taken from susceptible suspect animals to collect sufficient spatial and temporal descriptive data used to build the control programme. The national network for animal rabies surveillance is centralised at ANSES-Nancy laboratory for rabies and wildlife. The incidence of rabies in animals is currently assessed throughout all the country by undertaking diagnosis on suspect animals using the OIE and WHO reference tests, i.e. the fluorescent antibody test and the cell inoculation test (WHO, 2013; OIE, 2016). The positive cases are systematically investigated by sequencing to characterise the virus strains of isolated lyssaviruses, and also to study the circulating field isolates in the country. The monitoring of the efficacy of oral vaccination programmes was conducted in vaccinated areas by:.. Assessing bait uptake by testing the target species (foxes) in vaccinated areas for biomarker (tetracycline) occurrence and assessing age categories of foxes marked by tetracycline. Assessing rabies antibodies in target species in vaccinated areas: a cell virus neutralisaion method by using an ELISA previously validated for wildlife sample titration. ..   Technical competences in rabies .. The laboratory is experienced in the development validation and standardisation of laboratory methods, mainly for diagnosis and serology, and regularly organises international workshops and proficiency tests. The laboratory is enrolled in a system of quality assurance: Standard Test Accreditation body Accreditation date ISO/IEC1704 Rabies serological proficiency test COFRAC 05 May 2017 NF EN ISO/IEC 17025 FAT technique (Lyssavirus antigen) COFRAC 01 October 2012 NF EN ISO/IEC 17025 RTCIT technique (Lyssavirus) COFRAC 01 October 2012 NF EN ISO/IEC 17025 Control of efficacy of live oral vaccines in wildlife Mutual Joint Audit (EDQM) 30 January 2012 NF EN ISO/IEC 17025 Control of efficacy of inactivated rabies vaccines for veterinary and human use Mutual Joint Audit (EDQM) 05 March 2008 NF EN ISO/IEC 17025 Virus seroneutralisation (rabies antibody search) COFRAC 01 February 2008   The laboratory has developed the straw sampling technique to facilitate sampling on dead animals for diagnostic analysis. The laboratory has developed and validated a rapid, safe and inexpensive method for collecting brain samples from the field using filter paper (FTA technology) for further analyses by molecular techniques. Two different techniques have been developed which are currently recommended by WHO and OIE: An ELISA adapted to rabies antibody titration from wildlife field sera,  The FAVN test which is an adaptation of the RFFIT for specific detection of rabies neutralising antibodies in domestic and wild carnivores. A simple procedure has been recently developed by the laboratory allowing simple sampling of blood from foxes and raccoon dogs in field conditions coupled...

Training and development

Published on 26/05/2014

  The EURL provides scientific and technical assistance to all the NRLs .. Organisation of training session on request (in Nancy or in the NRL), with transfer of techniques, Organisation of workshops with interested NRLs on specific topics, Establishment of procedures and guidelines, Providing on-going remote technical assistance (phone meetings, e-mails, biological material supplied on request, etc.).     Techniques that could be validated and/or standardised within Member States:   Methods used for rabies diagnosis: Fluorescent Antibody Test (FAT), Rabies Tissue Culture Infection Test (RTCIT), Mouse Inoculation Test (MIT), Polymerase Chain Reaction (PCR) Method for determining the viral titre of the vaccine in the bait                                                                                                                                                                                                                                   Method for detecting tetracycline (or other          biomarker) in the teeth or bones of target           species killed in vaccinated areas Method for determining the age of animals         tested for biomarker determination  Methods for evaluating the stability of baits prior to use in the field Method used for fox serology (ELISA) of orally vaccinated animals Method for determining the potency of              inactivated vaccines   Last update: 08 August 2017