Reagents   The EURL can provide the following products: PRODUCT NAME CONDITIONNING RABIES DIAGNOSIS AND TYPING Reagent Polyclonal conjugate 3 ml (1vial per laboratory) Negative Control Non infected red fox brain 1 ml of lyophilised brain Fixed virus strain CVS 27 1 ml of lyophilised brain or 0.5 ml stabilized in RNA later Wild virus strains GS7 (French fox) 1 ml of lyophilised brain or 0.5 ml stabilized in RNA later Raccoon dog (Poland) 1 ml of lyophilised brain or 0.5 ml stabilized in RNA later Ariana (Tunisian dog) 1 ml of lyophilised brain or 0.5 ml stabilized in RNA later Imported dog strain (from Morocco) 1 ml of lyophilised brain or 0.5 ml stabilized in RNA later DUVV 1 ml of lyophilised brain or 0.5 ml stabilized in RNA later EBLV-1a 1 ml of lyophilised brain or 0.5 ml stabilized in RNA later EBLV-1b 1 ml of lyophilised brain or 0.5 ml stabilized in RNA later EBLV-2 1 ml of lyophilised brain or 0.5 ml stabilized in RNA later ABLV 1 ml of lyophilised brain or 0.5 ml stabilized in RNA later FOLLOW UP of ORAL VACCINATION: Age and tetracycline determination CD Rom Protocol Explanation Tooth sample Positive for tetracycline Tooth section or entire tooth Negative for tetracycline Tooth section or entire tooth OTHER Positive control CVS 11 750 µl of suspernatant lyophilised   Please contact the EURL for rabies for any order.   Last update: 09 August 2017 13/05/2019 1233 26/05/2014
Strain database presentation Strain database The EURL has developed a database recording epidemiological information on rabies strains including the corresponding Genbank sequence accession number. Linking Genbank numbers to this database will avoid the duplication of information and prevent redundancies for the NRLs since the GenBank database is an appropriate and useful tool already used for publication, research and analysis of sequences. The advantage of the EURL's sequence database is that it will make epidemiological information related to this sequence available. Submit cases Please participate actively in the deployment of the database by filling out the form for each of your new positive cases! Submit data on-line Download strain identification form (pdf, to be sent by mail or email) Consult data All data - strain database direct consultation Advanced search in database 06/08/2014 1232 26/05/2014
Contact Technopole Agricole et Vétérinaire de Pixérécourt Bâtiment H CS 40009 54220 MALZEVILLE FRANCE Phone: +33 (0)3 83 29 89 50 Fax: +33(0)3 83 29 89 58   For any questions or remarks regarding this website, or to suscribe to the newsletter, please contact us by email, specifying: “rabies” in the object your name, position and laboratory details in the message. Messages without “rabies” in the subject heading will be destroyed without being read. 13/05/2019 1212 26/05/2014
Map and directions   ANSES - Nancy laboratory for rabies and wildlife Laboratoire de la rage et de la faune sauvage de Nancy Technopole Agricole et Vétérinaire - Bâtiment H CS 40009 F-54220 MALZEVILLE FRANCE Phone: +33 (0)3 83 29 89 50 Fax: +33(0)3 83 29 89 58 Laboratory access map   13/05/2019 1211 26/05/2014
Links 05/06/2019 1210 26/05/2014
European Commission rules EURLs, what for? .. In the context of the EU strategy aimed at improving animal health and establishing the single market for live animals and animal products, a network of European Union and National Reference Laboratories dealing with major animal diseases has been gradually set up. The designation of European Union Reference Laboratories should contribute to a high quality and uniformity of analytical results. EURLs for animal health and zootechnics are listed in Annex VIII of Regulation (EC) No 882/2004 . Functions and duties of the EURLs consist notably of providing information on analysis methods and organising comparative tests with the National Reference Laboratories, coordinating the application of the methods and research into new analysis methods, organising training and advanced courses for National Reference Laboratory staff and providing scientific and technical assistance to the Commission. Apart from these duties and functions the Commission approves an annual working plan for each EURL specifying their tasks for the next year. .. Financial aid to EURLs .. Within the framework of Council Decision 90/424/EEC on expenditure in the veterinary field of 26 June 1990, these laboratories may receive European Union financial aid for fulfilling the tasks and functions specified in the animal health legislation. For this reason, the work programmes for the EURLs concerned are approved every year via specific Decisions, based on Council Decision 90/425/EEC. Appropriations shall be decided upon each year as part of the budgetary procedure. Commission Regulation (EC) No 1754/2006 of 28 November 2006 lays down detailed rules for the granting of European Union financial assistance to European Union Reference Laboratories for feed and food and the animal health sector pursuant to Article 28 of Decision 90/424/EEC. The said Regulation provides that the financial contribution from the European Union is to be granted if the approved work programmes are efficiently carried out and that the beneficiaries supply all the necessary information within certain time limits. In accordance with Article 2 of this Regulation, the relationship between the Commission and each European Union Reference Laboratory is laid down in a partnership agreement which is supported by a multi-annual work programme. .. EURL evaluation .. The main purpose of the evaluation is to assess the performance of the EURLs during the past 15 years, especially as regards effectiveness and efficiency and to identify areas for improvement to ensure high quality and harmonisation of laboratory testing of animal diseases across the Community, taking into account the current animal health situation in the EU. The EURL evaluation campaign was held in first quarter 2009. Since the rabies EURL was designated just before the evaluation campaign began (July 2008), it was not able to be evaluated. .. Eradication and monitoring programmes .. The European Commission has eradication and monitoring programmes for animal diseases. These programmes aim to progressively eliminate animal diseases that are endemic to certain areas of the European Union. They also include checks aimed to prevent zoonoses. The programmes include a wide range of measures including vaccination, testing of animals, compensation for slaughtering or culling and treatment. Rabies is part of the second group of diseases (“zoonoses or epizootics not covered elsewhere measures on a herd or flock basis”) covered by the EC programmes. .. Task force for monitoring disease eradication in the Member States .. The Task Force was created in a meeting held on 15 March 2000 in Brussels in accordance with action 29 of the White Paper on Food Safety . The objectives of the Task Force are to improve animal disease eradication as well as improve the cost-benefit ratio of animal disease eradication programmes co-financed by the European Union. Meetings are chaired by the Commission and take place in Brussels each three to six months. For some diseases (sheep and goat brucellosis, bovine brucellosis, bovine tuberculosis, rabies, salmonellosis) subgroups of the Task Force have been established. The subgroups support the Task Force and the Member States in their attempts to develop and to implement optimal disease eradication measures. The members of the subgroups are personally nominated. For each meeting of the Subgroups a final report is produced. In those reports, a number of conclusions and recommendations are provided. The Task Force for rabies was created in 2004. Year Date Town Country 2015 15-16 October Budapest Hungary 2012 11-12 December Warsaw Poland 2012 29-30 November Zagreb Croatia 2011 28 April Kaliningrad Russia 2011 14-15 March Plovdiv Bulgaria 2010 8-9... 13/05/2019 1209 26/05/2014
EU National Reference Laboratories for rabies List of the EU National Reference Laboratories for rabies, including presentation (for private members only) and official website:   AUSTRIA Presentation: Austrian NRL Official website: Austrian Agency for Health and Food Safety (AGES), Institute for Veterinary Disease Control   BELGIUM Presentation: Belgian NRL Official website: Sciensano   BULGARIA Presentation: Bulgarian NRL Laboratory: National Diagnostic Research Veterrinary Medical Institute (NDRVMI)   CROATIA (Republic of) Presentation: Croatian NRL Official website: Croatian Veterinary Institute   CYPRUS Presentation: Cypriot NRL Official website: Animal Health Laboratory - Virology section    CZECH REPUBLIC Presentation: Czech NRL Official website: State Veterinary Institute Prague         DENMARK     Presentation: Danish NRL Official website: DTU Vet    ESTONIA     Presentation: Estonian NRL Official website: Estonian veterinary and Food laboratory   FINLAND Presentation: Finnish NRL Official website: Finnish Food Safety Authority Evira    FRANCE     Presentation: French NRL and EURL Official website: Anses-Nancy Laboratory for rabies and wildlife    GERMANY Presentation: German NRL Official website: Friedrich-Loeffler-Institut - Federal Research Institute for Animal Health    GREECE Presentation: Greek NRL Laboratory: Virology Laboratory, Department of Molecular Diagnostics, FMD, Virological, Ricketsial and Exotic Diseases, Athens Veterinary Center    HUNGARY     Presentation: Hungarian NRL (sorry, no file) Official website: Veterinary Diagnostic Directorate, National Food Chain Safety Office    IRELAND Presentation: Irish NRL Official website: Central Veterinary Research Laboratory (CVRL), Department of Agriculture, Food and the Marine    ITALY Presentation: Italian NRL Official website: Istituto Zooprofilattico Sperimentale delle Venezie (IZSVe)    LATVIA Presentation: Latvian NRL Official website: Institute of Food Safety, Animal Health and Environment BIOR    LITHUANIA Presentation: Lithuanian NRL Official website: National Food and Veterinary Risk Assessment Institute    LUXEMBOURG Presentation: Luxembourg NRL (sorry, no file) Official website: Laboratoire de Medecine Veterinaire de L'Etat (in French)    MALTA Presentation: Maltese NRL (sorry, no file) Official website: Veterinary and Phytosanitary Regulation Department, Veterinary Regulation Directorate    THE NETHERLAND     Presentation: Dutch NRL Official website: Central Veterinary Institute of Wageningen UR    POLAND Presentation: Polish NRL Official website: National Veterinary Research Institute - Department of Virology    PORTUGAL      Presentation: Portuguese NRL (sorry, no file) Official website: INIAV, Instituto Nacional de Investigação Agrária e Veterinária, I.P. (In Portuguese)    ROMANIA     Presentation: Romanian NRL Official website: Institute for Diagnosis and Animal Health Bucharest - Virology Department (in Romanian)    SLOVAKIA     Presentation: Slovak NRL Official website: State Veterinary and Food Institute - Veterinary Institute Zvolen    SLOVENIA     Presentation: Slovenian NRL Official website: National veterinary institute    SPAIN     Presentation: Spanish NRL Official website: Instituto de Salud Carlos III, Centro Nacional de Microbiología    SWEDEN     Presentation: Swedish NRL Official website: SVA, National Veterinary Institute    UNITED KINGDOM Presentation: British NRL Official website: Animal & Plant Health Agency Weybridge     Last update: 08 August 2017 05/06/2019 1208 26/05/2014
Other international activities Last update: 20 November 2014   The laboratory is: WHO Collaborating Centre for Research and Management in zoonosis control since September 1983, OIE Reference Laboratory for rabies since June 1991, European Union Reference Laboratory for rabies serology since March 2000, European Union Reference Laboratory for rabies since July 2008. The laboratory provides scientific and technical support at the European level (European Commission, EDQM, EFSA), to international organizations (OIE, WHO) through numerous activities. The laboratory works closely to the National Reference Laboratories and their competent authorities for defining the strategy and the organization of oral vaccination programmes, as well as for establishing the surveillance and the monitoring of the disease (incidence of rabies, assessment of bait uptake and of rabies immunity both in adult and young target animals). The laboratory ensures training activities in the frame of its own programmes, within the limit of its possibilities and collaborates in the preparation and distribution of teaching materials. Biological reference material and laboratory services are available on request to help international laboratories to develop their own rabies surveillance and monitoring systems, as well as any other laboratory services. The rabies laboratory's experts provide scientific and technical assistance, advice and training for Member States and third countries. They are also involved in technical studies for improving and adapting existing diagnostic, serological, molecular biology and rpidemiological tools. ..     Additional documentation .. OIE  (World Organisation for Animal Health) WHO (World Health Organisation) PREDEMICS 13/05/2019 1204 26/05/2014
Work on rabies by Nancy laboratory for rabies and wildlife Organising, coordinating, implementing and monitoring oral fox vaccination campaigns against rabies .. In the 1970s, the laboratory extensively participated to scientific research work for developing oral vaccine baits. A number of experimental trials were undertaken to check efficacy and safety of vaccines before their marketing, in collaboration with other international groups involved in rabies activities. In 1986, the laboratory tested the first available methods of oral vaccination with baits (chicken head baits) deposited in the field by foot distribution. From 1990, the overall organisation of the oral vaccination campaigns has been centralised: the Anses-Nancy Laboratory for rabies and wildlife was requested by the national competent authority to organize and plan the oral vaccination of foxes and, in close cooperation with its delegates, to implement and evaluate the programmes. From 1988 to 1998, year of the last recorded case of vulpine rabies in France, the laboratory planned the strategy and performed oral vaccination campaigns in infected areas twice a year using helicopters. These campaigns were then prolonged until 2003 along Northern and Eastern borders and were performed once again in 2005. Several field trials have been conducted to improve the efficiency of oral vaccination programme, particularly to promote cubs' coming into contact with baits and also to counterbalance the increase in the fox population.   Rabies surveillance and vaccination monitoring .. Based on the collection of animals found dead, road killed, and suspect animals, as well as all animals having contamined humans: In case of human contamination (bite, scratch), animal cadavers are sent by the veterinary services to the Pasteur Institute. In all other cases, they are submitted by the veterinary services to Anses for rabies testing.   Rabies surveillance is the key element of rabies control and prevention programmes. It is based on laboratory investigations on samples taken from susceptible suspect animals to collect sufficient spatial and temporal descriptive data used to build the control programme. The national network for animal rabies surveillance is centralised at ANSES-Nancy laboratory for rabies and wildlife. The incidence of rabies in animals is currently assessed throughout all the country by undertaking diagnosis on suspect animals using the OIE and WHO reference tests, i.e. the fluorescent antibody test and the cell inoculation test (WHO, 2013; OIE, 2016). The positive cases are systematically investigated by sequencing to characterise the virus strains of isolated lyssaviruses, and also to study the circulating field isolates in the country. The monitoring of the efficacy of oral vaccination programmes was conducted in vaccinated areas by:.. Assessing bait uptake by testing the target species (foxes) in vaccinated areas for biomarker (tetracycline) occurrence and assessing age categories of foxes marked by tetracycline. Assessing rabies antibodies in target species in vaccinated areas: a cell virus neutralisaion method by using an ELISA previously validated for wildlife sample titration. ..   Technical competences in rabies .. The laboratory currently uses the folowing laboratory methods (Table 1): Table 1 Areas Methods Comments Diagnostic FAT RTCIT RIDT Conventional RT-PCR SYBR Green RT-PCR TaqMan RT-PCR     Serology FAVN test Indirect ELISA Competitive ELSA Used  on  animal  sera  and  animals immunoglobulins Vaccine potency testing NIH Pharmacopeia test TSA   Indirect ELISA Titration of rabies virus antibodies in wildlife samples for vaccination monitoring Cliquet et al., 2000 Monitoring of vaccination programmes Biomarker determination Age determination Both on tooth samples Virus titration         The laboratory is experienced in the development validation and standardisation of laboratory methods, mainly for diagnosis and serology, and regularly organises international workshops and proficiency tests.   The laboratory is enrolled in a system of quality assurance (Table 2): Table 2 Standard Test Accreditation body Accreditation date ISO/IEC1704 Rabies serological proficiency test COFRAC 05 May 2017 NF EN ISO/IEC 17025 FAT technique (Lyssavirus antigen) COFRAC 01 October 2012 NF EN ISO/IEC 17025 RTCIT technique (Lyssavirus) COFRAC 01 October 2012 NF EN ISO/IEC 17025 Control of efficacy of live oral vaccines... 05/06/2019 1203 26/05/2014
Training and development The EURL provides scientific and technical assistance to all the NRLs ●  Organisation of training session on request (in Nancy or in the NRL), with transfer of techniques, ●  Organisation of workshops with interested NRLs on specific topics, ●  Establishment of procedures and guidelines, ●  Providing on-going remote technical assistance (phone meetings, e-mails, biological material supplied on request, etc.). 05/06/2019 1202 26/05/2014